GFT projection NMR for efficient 1H/13C sugar spin system identification in nucleic acids
HS Atreya, B Sathyamoorthy, G Jaipuria… - Journal of biomolecular …, 2012 - Springer
HS Atreya, B Sathyamoorthy, G Jaipuria, V Beaumont, G Varani, T Szyperski
Journal of biomolecular NMR, 2012•SpringerA newly implemented G-matrix Fourier transform (GFT)(4, 3) D HC (C) CH experiment is
presented in conjunction with (4, 3) D HC CH to efficiently identify 1 H/13 C sugar spin
systems in 13 C labeled nucleic acids. This experiment enables rapid collection of highly
resolved relay 4D HC (C) CH spectral information, that is, shift correlations of 13 C–1 H
groups separated by two carbon bonds. For RNA,(4, 3) D HC (C) CH takes advantage of the
comparably favorable 1′-and 3′-CH signal dispersion for complete spin system …
presented in conjunction with (4, 3) D HC CH to efficiently identify 1 H/13 C sugar spin
systems in 13 C labeled nucleic acids. This experiment enables rapid collection of highly
resolved relay 4D HC (C) CH spectral information, that is, shift correlations of 13 C–1 H
groups separated by two carbon bonds. For RNA,(4, 3) D HC (C) CH takes advantage of the
comparably favorable 1′-and 3′-CH signal dispersion for complete spin system …
Abstract
A newly implemented G-matrix Fourier transform (GFT) (4,3)D HC(C)CH experiment is presented in conjunction with (4,3)D HCCH to efficiently identify 1H/13C sugar spin systems in 13C labeled nucleic acids. This experiment enables rapid collection of highly resolved relay 4D HC(C)CH spectral information, that is, shift correlations of 13C–1H groups separated by two carbon bonds. For RNA, (4,3)D HC(C)CH takes advantage of the comparably favorable 1′- and 3′-CH signal dispersion for complete spin system identification including 5′-CH. The (4,3)D HC(C)CH/HCCH based strategy is exemplified for the 30-nucleotide 3′-untranslated region of the pre-mRNA of human U1A protein.
Springer
以上显示的是最相近的搜索结果。 查看全部搜索结果